Nutrient agar. 18 * Table 3.4 Differential media α, β and γ haemolysis) Example of selective media supplemented with dyes: Crystal violet agar medium comprises 0.0002% CV that helps in the isolation of Streptococcus pyogenes. A differential medium support the growth of any microbe but distinguishes them based on how they metabolize or change the medium. Enriched media selects for a certain group of microbes while an enrichment media selects for one microbe. It is not selective, and will grow many different types of microbes. On this MacConkey agar plate, the lactose-fermenter E. coli colonies are bright pink. S.                               pyogenes, S. agalactiae, and several other species of streptococci are β hemolytic. Staphylococcus and Enterobacteriaceae grow in these media. Differential media are bacteriological growth media that contain specific ingredients to allow one to distinguish selected species or categories of bacteria by visual observation. Hemolysis Observation of the hemolytic reactions on blood agar is a very useful tool in the identification of bacteria, particularly streptococci. Examples of differential media include: Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic. Enriched media contain the nutrients required to support the growth of a wide variety of organisms, including some fastidious ones. MacConkey agar is an example of a medium that is both differential and selective. Selective and Differential Media. Conferences, About Us | Contact | FAQ | Manufacturer Login, All Categories 1. East Lansing, MI 48824, Site Design by B-CLR and Academic Technology. 3.7 Examples of enriched media 17 " # ' Fig. Blood agar contains 5-10% blood by volume to a blood agar base. - Click to open the module Pre-reduced anaerobically sterilized plated media for the isolation, quantitation and partial identi... Pre-reduced anaerobically sterilized plated media for the isolation and cultivation of fastidious an... 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Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups. DE's are mechanistic models, where we define the … 16 * Fig. For example, MacConkey's agar differentiates ENTEROBACTERIA that ferment LACTOSE from non-lactose fermenters (NLFs). Figure 1. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. beta (β) hemolysis: A clear, colorless zone appears around the colonies, in which the RBCs have undergone complete lysis. Chemical Detection Examples of differential media include: Mannitol salts agar (mannitol fermentation = yellow) Blood agar (various kinds of hemolysis i.e. Selective media is often used when one is trying to isolate a specimen of interest from a mixed culture. Due to the presence of certain dyes or chemicals in the media, the organisms will produce characteristic changes or growth patterns that are used for identification or differentiation. Biological Detection BASAL MEDIA. A medium may be enriched, i.e., by the addition of blood or serum. Chemical Detection They are commonly used to grow as many different types of microbes as are present in the specimen. Differential Media are media that distinguish between groups of bacteria and even permit tentative identification of micro-organisms based on their biological characteristics. Differential media allow the growth of more than one microorganism of interest but with morphologically distinguishable colonies. Examples: EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) are examples of selective media. The medium contains mannitol, a phenol red indicator, and 7.5% sodium chloride. Some differential media are also selective, for example, standard enteric agars such as MacConkey and EMB agars, which are selective for gram-negative coliforms and can differentiate lactose-fermenting and non-lactose-fermenting bacteria. Radiological/Nuclear Detection One example of differential medium is blood agar. Final Exam Intro to Microbiology Status: Not Started. The difference between selective and differential media is mainly due to the following factors: Intended use of the media: The purpose of both selective and differential media differs.A selective media only permits the development of particular organisms, while a differential media discerns one species of the organism from the other growing on the similar substrate. Three of the more common selective and differential media are described below and will be used in the laboratory exercise. The presence of bile salts, as well as crystal violet, within the media prevent gram-positive organisms from … no (γ) hemolysis: No apparent hemolytic activity or discoloration is produced by the colony (also called gamma hemolysis). α, β and γ hemolysis) Differential media contain compounds that allow some groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media. Anaerobe Systems (2) For example in case of Mannitol salt agar, it contains mannitol and phenol red as pH indicator which support the growth of mannitol fermenting staphylococcus . Colonies of the lactose fermenters are red or pink, while those of NLFs are colourless. Explosives Detection Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups. Selective media contain ingredients that inhibit the growth of some organisms but allow others to grow. For example, mannitol salt agar contains a high concentration of sodium chloride that inhibits the growth of most organisms but permits staphylococci to grow. Reset Filters. Nutrient broth, tryptic soy broth, and brain heart infusion, are all examples of complex media. Differential media are used to differentiate closely related organisms or groups of organisms. Explore examples of selective and differential media; Practice Exams. Explosives Detection, Home > Biological > Technology > Cell Culture > Differential Media. Complex, non-selective, non-differential. a media which differentiates or distinguishes between different types of microorganisms based on differences in appearance of growth or color changes. Examples of standard general purpose media that will support the growth of a wide variety of bacteria include nutrient agar, tryptic soy agar, and brain heart infusion agar. 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